Development and Evaluation of ¹⁸F-IRS for Molecular Imaging Mutant EGF Receptors in NSCLC.

ABSTRACT: To prepare and evaluate a new radiotracer ¹⁸F-IRS for molecular imaging mutant EGF Receptors in vitro and vivo. Uptake and efflux of ¹⁸F-IRS were performed with four NSCLC cell lines including HCC827, H1975, H358 and H520. In vivo tumor targeting and pharmacokinetics of the radiotracers were also evaluated in HCC827, H1975, H358 and H520 tumor-bearing nude mice by PET/CT imaging. Ex vivo biodistribution assays were performed to quantify the accumulation of ¹⁸F-IRS in vivo. We also performed ¹⁸F-IRS PET/CT imaging of three patients with NSCLC. We labeled this small molecule with QD620 for flow cytometry and confocal imaging analyses. The uptakes of ¹⁸F-IRS by HCC827 and HCC827 tumors were significantly higher than those of H358, H1975 a

SUBMITTER: Song Y 

PROVIDER: S-EPMC5466683 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature