Unknown

Dataset Information

0

Integrative Proteomics and Phosphoproteomics Profiling Reveals Dynamic Signaling Networks and Bioenergetics Pathways Underlying T Cell Activation.


ABSTRACT: The molecular circuits by which antigens activate quiescent T cells remain poorly understood. We combined temporal profiling of the whole proteome and phosphoproteome via multiplexed isobaric labeling proteomics technology, computational pipelines for integrating multi-omics datasets, and functional perturbation to systemically reconstruct regulatory networks underlying T cell activation. T cell receptors activated the T cell proteome and phosphoproteome with discrete kinetics, marked by early dynamics of phosphorylation and delayed ribosome biogenesis and mitochondrial activation. Systems biology analyses identified multiple functional modules, active kinases, transcription factors and connectivity between them, and mitochondrial pathways including mitoribosomes and complex IV. Genetic perturbation revealed physiological roles for mitochondrial enzyme COX10-mediated oxidative phosphorylation in T cell quiescence exit. Our multi-layer proteomics profiling, integrative network analysis, and functional studies define landscapes of the T cell proteome and phosphoproteome and reveal signaling and bioenergetics pathways that mediate lymphocyte exit from quiescence.

SUBMITTER: Tan H 

PROVIDER: S-EPMC5466820 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Integrative Proteomics and Phosphoproteomics Profiling Reveals Dynamic Signaling Networks and Bioenergetics Pathways Underlying T Cell Activation.

Tan Haiyan H   Yang Kai K   Li Yuxin Y   Shaw Timothy I TI   Wang Yanyan Y   Blanco Daniel Bastardo DB   Wang Xusheng X   Cho Ji-Hoon JH   Wang Hong H   Rankin Sherri S   Guy Cliff C   Peng Junmin J   Chi Hongbo H  

Immunity 20170309 3


The molecular circuits by which antigens activate quiescent T cells remain poorly understood. We combined temporal profiling of the whole proteome and phosphoproteome via multiplexed isobaric labeling proteomics technology, computational pipelines for integrating multi-omics datasets, and functional perturbation to systemically reconstruct regulatory networks underlying T cell activation. T cell receptors activated the T cell proteome and phosphoproteome with discrete kinetics, marked by early d  ...[more]

Similar Datasets

| S-EPMC6901481 | biostudies-literature
| S-EPMC7706838 | biostudies-literature
2017-03-29 | PXD005492 | Pride
2017-03-15 | PXD004367 | Pride
| S-EPMC8419860 | biostudies-literature
| S-EPMC6398214 | biostudies-literature
| S-EPMC4812528 | biostudies-literature
| S-EPMC2728537 | biostudies-literature
| S-EPMC2637050 | biostudies-literature
| S-EPMC8584006 | biostudies-literature