Identification of clear cell renal cell carcinoma and oncocytoma using a three-gene promoter methylation panel.

ABSTRACT:

Background

Promoter methylation has emerged as a promising class of epigenetic biomarkers for diagnosis and prognosis of renal cell tumors (RCTs). Although differential gene promoter methylation patterns have been reported for the major subtypes (clear cell, papillary and chromophobe renal cell carcinoma, and oncocytoma), validation of diagnostic performance in independent series have been seldom performed. Herein, we aimed at assessing the diagnostic performance of genes previously shown to be hypermethylated in RCTs in different clinical settings.

Methods

Promoter methylation levels of HOXA9 and OXR1 were assessed by quantitative methylation specific PCR. ROC curves were generated for OXR1, OXR1 combined with MST1R and HOXA9. Sensitivity, specificity, positive predictive v

SUBMITTER: Pires-Luis AS 

PROVIDER: S-EPMC5492907 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature