Genetic dissociation of morphine analgesia from hyperalgesia in mice.

ABSTRACT:

Rationale

Morphine is the prototypic mu opioid, producing its analgesic actions through traditional 7 transmembrane domain (7TM) G-protein-coupled receptors generated by the mu opioid receptor gene (Oprm1). However, the Oprm1 gene undergoes extensive alternative splicing to yield three structurally distinct sets of splice variants. In addition to the full-length 7TM receptors, it produces a set of truncated variants comprised of only 6 transmembrane domains (6TM).

Objectives

This study explored the relative contributions of 7TM and 6TM variants in a range of morphine actions.

Methods

Groups of male and mixed-gender wild-type and exon 11 Oprm1 knockout mice were examined in a series of behavioral assays measuring analgesia, hyperalgesia, respiration, and reward in cond

SUBMITTER: Marrone GF 

PROVIDER: S-EPMC5520541 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature