Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections.

ABSTRACT: Frozen sections (FS) of tumor samples represent a cornerstone of pathological intraoperative consultation and have an important role in the microscopic analysis of specimens during surgery. So far, immunohistochemical (IHC) stainings on FS have been demonstrated for a few markers using manual methods. Microfluidic technologies have proven to bring substantial improvement in many fields of diagnostics, though only a few microfluidic devices have been designed to improve the performance of IHC assays. In this work, we show optimization of a complete pan-cytokeratin chromogenic immunostaining protocol on FS using a microfluidic tissue processor into a protocol taking <12 min. Our results showed specificity and low levels of background. The dimensions of the microfluidic prototype device are c

SUBMITTER: Brajkovic S 

PROVIDER: S-EPMC5533214 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature