Stable engraftment of human microbiota into mice with a single oral gavage following antibiotic conditioning.

ABSTRACT:

Background

Human microbiota-associated (HMA) animal models relying on germ-free recipient mice are being used to study the relationship between intestinal microbiota and human disease. However, transfer of microbiota into germ-free animals also triggers global developmental changes in the recipient intestine, which can mask disease-specific attributes of the donor material. Therefore, a simple model of replacing microbiota into a developmentally mature intestinal environment remains highly desirable.

Results

Here we report on the development of a sequential, three-course antibiotic conditioning regimen that allows sustained engraftment of intestinal microorganisms following a single oral gavage with human donor microbiota. SourceTracker, a Bayesian, OTU-based algorithm, indi

SUBMITTER: Staley C 

PROVIDER: S-EPMC5537947 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature