Biochemical phosphates observed using hyperpolarized ³¹P in physiological aqueous solutions.

ABSTRACT: The dissolution-dynamic nuclear polarization technology had previously enabled nuclear magnetic resonance detection of various nuclei in a hyperpolarized state. Here, we show the hyperpolarization of ³¹P nuclei in important biological phosphates (inorganic phosphate and phosphocreatine) in aqueous solutions. The hyperpolarized inorganic phosphate showed an enhancement factor >11,000 (at 5.8 T, 9.3% polarization) in D₂O (T₁ 29.4 s). Deuteration and the solution composition and pH all affected the lifetime of the hyperpolarized state. This capability opens up avenues for real-time monitoring of phosphate metabolism, distribution, and pH sensing in the live body without ionizing radiation. Immediate changes in the microenvironment pH have been detected here in

SUBMITTER: Nardi-Schreiber A 

PROVIDER: S-EPMC5570947 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature