Histone locus regulation by the Drosophila dosage compensation adaptor protein CLAMP.

ABSTRACT: The conserved histone locus body (HLB) assembles prior to zygotic gene activation early during development and concentrates factors into a nuclear domain of coordinated histone gene regulation. Although HLBs form specifically at replication-dependent histone loci, the cis and trans factors that target HLB components to histone genes remained unknown. Here we report that conserved GA repeat cis elements within the bidirectional histone3-histone4 promoter direct HLB formation in Drosophila In addition, the CLAMP (chromatin-linked adaptor for male-specific lethal [MSL] proteins) zinc finger protein binds these GA repeat motifs, increases chromatin accessibility, enhances histone gene transcription, and promotes HLB formation. We demonstrated previously that

SUBMITTER: Rieder LE 

PROVIDER: S-EPMC5588930 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature