Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis.

ABSTRACT:

Background and purpose

Hyphal wall protein 1 (HWP1) is an important adhesin which usually is expressed on the germ tube and hyphal surface produced by different Candida species. The hyphal wall protein-coding gene (HWP1) was evaluated as a novel identification and phylogenetic marker in Candida tropicalis, C. orthopsilosis, C. parapsilosis and C. glabrata.

Materials and methods

Initially, four specific primer pairs were designed, and the target was amplified and finally sequenced. A total of 77 Candida isolates from four different species were included in the study. Consensus sequences were used for the evaluation of phylogenetic tree using the CLC Genome Workbench, GENEIOUS, and MEGA softwares and the levels of n

SUBMITTER: Abastabar M 

PROVIDER: S-EPMC5611690 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature