Copper homeostasis networks in the bacterium Pseudomonas aeruginosa.

ABSTRACT: Bacterial copper (Cu⁺) homeostasis enables both precise metallation of diverse cuproproteins and control of variable metal levels. To this end, protein networks mobilize Cu⁺ to cellular targets with remarkable specificity. However, the understanding of these processes is rather fragmented. Here, we use genome-wide transcriptomic analysis by RNA-Seq to characterize the response of Pseudomonas aeruginosa to external 0.5 mm CuSO₄, a condition that did not generate pleiotropic effects. Pre-steady-state (5-min) and steady-state (2-h) Cu⁺ fluxes resulted in distinct transcriptome landscapes. Cells quickly responded to Cu²⁺ stress by slowing down metabolism. This was restored once steady state was reached. Specific Cu⁺ homeo

SUBMITTER: Quintana J 

PROVIDER: S-EPMC5612103 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature