Metabolomic screening using ESI-FT MS identifies potential radiation-responsive molecules in mouse urine.

ABSTRACT: The demand for establishment of high-throughput biodosimetric methods is increasing. Our aim in this study was to identify low-molecular-weight urinary radiation-responsive molecules using electrospray ionization Fourier transform mass spectrometry (ESI-FT MS), and our final goal was to develop a sensitive biodosimetry technique that can be applied in the early triage of a radiation emergency medical system. We identified nine metabolites by statistical comparison of mouse urine before and 8 h after irradiation. Time-course analysis showed that, of these metabolites, thymidine and either thymine or imidazoleacetic acid were significantly increased dose-dependently 8 h after radiation exposure; these molecules have already been reported as potential radiation biomarkers. Phenyl glucuronide

SUBMITTER: Iizuka D 

PROVIDER: S-EPMC5619916 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature