Identification of reference genes in blood before and after entering the plateau for SYBR green RT-qPCR studies.

ABSTRACT:

Background

Tibetans have lived at high altitudes for thousands of years, and they have unique physiological traits that enable them to tolerate this hypoxic environment. However, the genetic basis of these traits is still unknown. As a sensitive and highly efficient technique, RT-qPCR is widely used in gene expression analyses to provide insight into the molecular mechanisms underlying environmental changes. However, the quantitative analysis of gene expression in blood is limited by a shortage of stable reference genes for the normalization of mRNA levels. Thus, systematic approaches were used to identify potential reference genes.

Results

The expression levels of eight candidate human reference genes (GAPDH, ACTB, 18S RNA, β2-MG, PPIA<

SUBMITTER: Xiao J 

PROVIDER: S-EPMC5622608 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature