Dataset Information

Comparative characterization of bacterial communities in geese fed all-grass or high-grain diets.

ABSTRACT:

Background

Gut microbial composition is dependent on diet. Geese are herbivores and can digest crude fibre, but the relationship between composition of the microbiota and a fibre-rich diet in geese is not well understood.

Results

Here, caecal and faecal samples were collected simultaneously from all-grass-fed geese and high-grain-fed geese and the hypervariable V3-V4 regions of the bacterial 16S rRNA gene were sequenced. The results was identified that high-grass-fed geese possessed significantly higher alpha diversity both in caecum and faeces compared with that in all-grain-fed geese. In addition, the composition of dominant bacterium occurred remarkable shifting due to different diet patterns, Firmicutes were more abundant in all-grass-fed geese, whereas Bacteroidetes were abundant in high-grain-fed geese. Fusobacteria and Deferribacteres were obviously present in high-grain-fed geese and few in all-grass-fed geese. Most importantly, some specific microorgnisms such as Ruminococcaceae, Lachnospiraceae and Bacteroidaceae which may associated with cellulose-degrading that were characterized to show distinctly diverse between the two diet patterns. PICRUSt analysis revealed the metabolic pathways such as carbohydrate and amino acid metabolism were overrepresented in all-grass-fed geese.

Conclusions

In conclusion, Firmicutes and Bacteroidetes were identified abundantly when the geese was fed with all-grass feed and high-grain feed, respectively. And Ruminococcaceae, Lachnospiraceae and Bacteroidaceae were recognized as main cellulose-degrading bacteria in the geese. The functional profiles of gut microbiota revealed the dominant microbiota communities were involved mainly in the carbohydrate metabolism in all-grass-fed geese.

SUBMITTER: Xu Q

PROVIDER: S-EPMC5626430 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Comparative characterization of bacterial communities in geese fed all-grass or high-grain diets.

Xu Qi Q Yuan Xiaoya X Gu Tiantian T Li Yang Y Dai Wangcheng W Shen Xiaokun X Song Yadong Y Zhang Yang Y Zhao Wenming W Chang Guobin G Chen Guohong G

PloS one 20171003 10

<h4>Background</h4>Gut microbial composition is dependent on diet. Geese are herbivores and can digest crude fibre, but the relationship between composition of the microbiota and a fibre-rich diet in geese is not well understood.<h4>Results</h4>Here, caecal and faecal samples were collected simultaneously from all-grass-fed geese and high-grain-fed geese and the hypervariable V3-V4 regions of the bacterial 16S rRNA gene were sequenced. The results was identified that high-grass-fed geese possess ...[more]

PMID: 28972993

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Project description:Beef represents a major diet component and one of the major sources of protein in human. The beef industry in the United States is currently undergoing changes and is facing increased demands especially for natural grass-fed beef. The grass-fed beef obtained their nutrients directly from pastures, which contained limited assimilable energy but abundant amount of fiber. On the contrary, the grain-fed steers received a grain-based regime that served as an efficient source of high-digestible energy. Lately, ruminant animals have been accused to be a substantial contributor for the green house effect. Therefore, the concerns from environmentalism, animal welfare and public health have driven consumers to choose grass-fed beef. Rumen is one of the key workshops to digest forage constituting a critical step to supply enough nutrients for animals’ growth and production. We hypothesize that rumen may function differently in grass- and grain-fed regimes. The objective of this study was to find the differentially expressed genes in the ruminal wall of grass-fed and grain-fed steers, and then explore the potential biopathways. In this study, the RNA Sequencing (RNA-Seq) method was used to measure the gene expression level in the ruminal wall. The total number of reads per sample ranged from 24,697,373 to 36,714,704. The analysis detected 342 differentially expressed genes between ruminal wall samples of animals raised under different regimens. The Fisher’s exact test performed in the Ingenuity Pathway Analysis (IPA) software found 16 significant molecular networks. Additionally, 13 significantly enriched pathways were identified, most of which were related to cell development and biosynthesis. Our analysis demonstrated that most of the pathways enriched with the differentially expressed genes were related to cell development and biosynthesis. Our results provided valuable insights into the molecular mechanisms resulting in the phenotype difference between grass-fed and grain-fed cattle.

Dataset Information

Comparative characterization of bacterial communities in geese fed all-grass or high-grain diets.

Background

Results

Conclusions

Publications

Comparative characterization of bacterial communities in geese fed all-grass or high-grain diets.

Similar Datasets

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