A TIM-3 Oligonucleotide Aptamer Enhances T Cell Functions and Potentiates Tumor Immunity in Mice.

ABSTRACT: T cell immunoglobulin-3 (TIM-3) is a negative regulator of interferon-γ (IFN-γ) secreting CD4⁺ T cells and CD8⁺ T cytotoxic cells. Recent studies have highlighted the role of TIM-3 as an important mediator of CD8⁺ T cell exhaustion in the setting of chronic viral infections and cancer. In murine tumor models, antibody blockade of TIM-3 with anti-TIM-3 antibodies as monotherapy has no or minimal antitumor activity, suggesting that TIM-3 signaling exerts an accessory or amplifying effect in keeping immune responses in check. Using a combined bead and cell-based systemic evolution of ligands by exponential enrichment (SELEX) protocol, we have isolated nuclease-resistant oligonucleotide aptamer ligands that bind to cell-associated TIM-3 with high affinity and s

SUBMITTER: Gefen T 

PROVIDER: S-EPMC5628791 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature