Dataset Information

Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy.

ABSTRACT:

Purpose

To deliver insoluble natural compounds into colon cancer cells in a controlled fashion.

Materials and methods

Curcumin (CM)-silk fibroin (SF) nanoparticles (NPs) were prepared by solution-enhanced dispersion by supercritical CO₂ (SEDS) (20 MPa pressure, 1:2 CM:SF ratio, 1% concentration), and their physicochemical properties, intracellular uptake efficiency, in vitro anticancer effect, toxicity, and mechanisms were evaluated and analyzed.

Results

CM-SF NPs (<100 nm) with controllable particle size were prepared by SEDS. CM-SF NPs had a time-dependent intracellular uptake ability, which led to an improved inhibition effect on colon cancer cells. Interestingly, the anticancer effect of CM-SF NPs was improved, while the side effect on normal human colon mucosal epithelial cells was reduced by a concentration of ~10 μg/mL. The anticancer mechanism involves cell-cycle arrest in the G₀/G₁ and G₂/M phases in association with inducing apoptotic cells.

Conclusion

The natural compound-loaded SF nanoplatform prepared by SEDS indicates promising colon cancer-therapy potential.

SUBMITTER: Xie M

PROVIDER: S-EPMC5659230 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Publications

Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy.

Xie Maobin M Fan Dejun D Li Yi Y He Xiaowen X Chen Xiaoming X Chen Yufeng Y Zhu Jixiang J Xu Guibin G Wu Xiaojian X Lan Ping P

International journal of nanomedicine 20171020

<h4>Purpose</h4>To deliver insoluble natural compounds into colon cancer cells in a controlled fashion.<h4>Materials and methods</h4>Curcumin (CM)-silk fibroin (SF) nanoparticles (NPs) were prepared by solution-enhanced dispersion by supercritical CO<sub>2</sub> (SEDS) (20 MPa pressure, 1:2 CM:SF ratio, 1% concentration), and their physicochemical properties, intracellular uptake efficiency, in vitro anticancer effect, toxicity, and mechanisms were evaluated and analyzed.<h4>Results</h4>CM-SF NP ...[more]

PMID: 29118580

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Project description:Mutual diffusion of six hydrocarbons (methane, ethane, isobutane, benzene, toluene or naphthalene) diluted in supercritical carbon dioxide ([Formula: see text]) is studied by molecular dynamics simulation near the Widom line, i.e., in the temperature range from 290 to 345 K along the isobar 9 MPa. The [Formula: see text] + aromatics mixtures are additionally sampled at 10 and 12 MPa and an experimental database with Fick diffusion coefficient data for those systems is provided. Taylor dispersion experiments of [Formula: see text] with benzene, toluene, n-dodecane and 1,2,3,4-tetrahydronaphthalene are conducted along the [Formula: see text] 10 MPa isobar. Maxwell-Stefan and Fick diffusion coefficients are analyzed, together with the thermodynamic factor that relates them. It is found that the peculiar behavior of the Fick diffusion coefficient of some [Formula: see text] mixtures in the extended critical region is a consequence of the thermodynamic factor minimum due to pronounced clustering on the molecular scale. Further, the strong dependence of the Fick diffusion coefficient on the molecular mass of the solute as well as the breakdown of the Stokes-Einstein relation near the Widom line are confirmed. Eleven correlations for the prediction of the Fick diffusion coefficient of [Formula: see text] mixtures are assessed. An alternative two-step approach for the prediction of the infinite dilution Fick diffusion coefficient of supercritical [Formula: see text] mixtures is proposed. It requires only the state point in terms of temperature and pressure (or density) as well as the molecular solute mass as input parameters. First, entropy scaling is applied to estimate the self-diffusion coefficient of [Formula: see text]. Subsequently, this coefficient is used to determine the infinite dilution Fick diffusion coefficient of the mixture, based on the finding that these two diffusion coefficients exhibit a linear relationship, where the slope depends only on the molecular solute mass.

Dataset Information

Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy.

Purpose

Materials and methods

Results

Conclusion

Publications

Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy.

Similar Datasets

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