Ontology highlight
ABSTRACT:
SUBMITTER: Awai T
PROVIDER: S-EPMC5668874 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Awai Takako T Ichihashi Norikazu N Yomo Tetsuya T
Biochemistry and biophysics reports 20150808
A significant challenge in the field of in vitro synthetic biology is the construction of a self-reproducing cell-free translation system, which reproduces its components, such as translation proteins, through translation and transcription by itself. As a first step for such construction, in this study we expressed and evaluated the activity of 20 aminoacyl-tRNA synthetases (aaRSs), a major component of a translation system, in a reconstituted translation system (PURE system). We found that 19 a ...[more]