Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.

ABSTRACT: The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe "semisynthetic" pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.

SUBMITTER: Martineau M 

PROVIDER: S-EPMC5680258 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature