Estrogen attenuates AGTR1 expression to reduce pancreatic β-cell death from high glucose.

ABSTRACT: Chronic exposure of pancreatic β-cells to high glucose levels results in β-cell dysfunction and death. These effects can be protected by estrogen. The local pancreatic renin-angiotensin system (RAS) has been shown as a novel pathological pathway of high-glucose-induced cell death. The effect of estrogen on pancreatic RAS is still unknown. This study examines whether estrogen protects against pancreatic β-cell death caused by glucotoxicity via a decrease in the pancreatic β-cell RAS pathway. When INS-1 cells were cultured in a high glucose medium, cell death was significantly higher than when the cells were cultured in a basal glucose medium; similarly, there were also higher levels of AGTR1 and p47 ph ° x mRNA, and protein expression. Moreover, the addition of 10-8 M 17β-estradiol to INS-1

SUBMITTER: Kooptiwut S 

PROVIDER: S-EPMC5709427 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature