Blood collection in cell-stabilizing tubes does not impact germline DNA quality for pediatric patients.

ABSTRACT:

Objectives

Liquid biopsy technologies allow non-invasive tumor profiling for patients with solid tumor malignancies by sequencing circulating tumor DNA. These studies may be useful in risk-stratification, monitoring for relapse, and understanding tumor evolution. The quality of DNA obtained for these studies is improved when blood samples are collected in tubes that stabilizing white blood cells (WBC). However, ongoing germline research in pediatric oncology generally requires obtaining blood samples in EDTA tubes, which do not contain a WBC-stabilizing preservative. In this study, we explored whether blood samples collected in WBC-stabilizing tubes could be used for both liquid biopsy and germline studies simultaneously, minimizing blood collection volumes for pediatric patients.<

SUBMITTER: Wollison BM 

PROVIDER: S-EPMC5716571 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature