Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli.

ABSTRACT: In order to identify Trichinella at the species level, the commonly used test is a multiplex PCR, allowing the discrimination of nine out of the twelve taxa described so far. This test is based on five primer pairs amplifying fragments of the large subunit rDNA. Each taxon produces one or two bands of different sizes, resulting in a specific band pattern. By multiplex PCR, Trichinella?murrelli shows two bands of 127?bp and 316?bp. However, a third band of 256?bp can occur. This band can lead to misidentification, since it is similar to the 253?bp band displayed by Trichinella?britovi. BLAST analysis confirmed that the 256?bp band is from T. murrelli. The aim of this short note is to inform analysts that T.?murrelli larvae may display either two- or three-band patterns.

SUBMITTER: Karadjian G 

PROVIDER: S-EPMC5721686 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature