Detection and sequencing of rotavirus among sudanese children.

ABSTRACT:

Introduction

Diarrheal diseases are a big public health problem worldwide, particularly among developing countries. The current study was conducted to detect and characterize group A rotavirus among admitted children with gastroenteritis to the pediatric hospitals, Sudan.

Methods

A total of 755 stool samples were collected from Sudanese children with less than 5 years of age presenting with acute gastroenteritis during the period from April to September 2010. Enzyme-linked immunosorbent assay (ELISA) was used to Detection of Rotavirus antigens. Ribonucleic acid (RNAs) were extracted from rotavirus-positive stool samples using (QIAamp^® Viral RNA Mini Kit). (Omniscript^® Reverse Transcription kit) was used to convert RNA to complementary Deoxyribonucleic a

SUBMITTER: Magzoub MA 

PROVIDER: S-EPMC5724952 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature