Platelet protein biomarker panel for ovarian cancer diagnosis.

ABSTRACT:

Background

Platelets support cancer growth and spread making platelet proteins candidates in the search for biomarkers.

Methods

Two-dimensional (2D) gel electrophoresis, Partial Least Squares Discriminant Analysis (PLS-DA), Western blot, DigiWest.

Results

PLS-DA of platelet protein expression in 2D gels suggested differences between the International Federation of Gynaecology and Obstetrics (FIGO) stages III-IV of ovarian cancer, compared to benign adnexal lesions with a sensitivity of 96% and a specificity of 88%. A PLS-DA-based model correctly predicted 7 out of 8 cases of FIGO stages I-II of ovarian cancer after verification by western blot. Receiver-operator curve (ROC) analysis indicated a sensitivity of 83% and specificity of 76% at cut-off >0.5 (area under the

SUBMITTER: Lomnytska M 

PROVIDER: S-EPMC5767003 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature