Native elongating transcript sequencing reveals global anti-correlation between sense and antisense nascent transcription in fission yeast.

ABSTRACT: Antisense transcription can regulate sense gene expression. However, previous annotations of antisense transcription units have been based on detection of mature antisense long noncoding (aslnc)RNAs by RNA-seq and/or microarrays, only giving a partial view of the antisense transcription landscape and incomplete molecular bases for antisense-mediated regulation. Here, we used native elongating transcript sequencing to map genome-wide nascent antisense transcription in fission yeast. Strikingly, antisense transcription was detected for most protein-coding genes, correlating with low sense transcription, especially when overlapping the mRNA start site. RNA profiling revealed that the resulting aslncRNAs mainly correspond to cryptic Xrn1/Exo2-sensitive transcripts (XUTs). ChIP-seq analyses sho

SUBMITTER: Wery M 

PROVIDER: S-EPMC5769747 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature