Triggering molecular assembly at the mesoscale for advanced Raman detection of proteins in liquid.

ABSTRACT: An advanced optofluidic system for protein detection based on Raman signal amplification via dewetting and molecular gathering within temporary mesoscale assemblies is presented. The evaporation of a microliter volume of protein solution deposited in a circular microwell precisely follows an outward-receding geometry. Herein the combination of liquid withdrawal with intermolecular interactions induces the formation of self-assembled molecular domains at the solid-liquid interface. Through proper control of the evaporation rate, amplitude of the assemblies and time for spectral collection at the liquid edge are extensively raised, resulting in a local enhancement and refinement of the Raman response, respectively. Further signal amplification is obtained by taking advantage of the intense l

SUBMITTER: Banchelli M 

PROVIDER: S-EPMC5773671 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature