Mechanistic insights into the detection of free fatty and bile acids by ileal glucagon-like peptide-1 secreting cells.

ABSTRACT:

Objectives

The aim of this study was to investigate the electrical properties of ileal Glucagon-like peptide 1 (GLP-1) secreting L-cells using murine organoid cultures and the electrophysiological and intracellular signaling pathways recruited following activation of the G_αq-coupled free fatty acid receptors FFA1 and G_αs-coupled bile acid receptors GPBAR1.

Methods

Experiments were performed using ileal organoids generated from mice transgenically expressing fluorescent reporters (Epac2-camps and GCaMP3) under control of the proglucagon promoter. Electrophysiology and single cell imaging were performed on identified L-cells in organoids, and GLP-1 secretion from cultured organoids was measured by immunoassay.

Results

The FFA1 ligand TAK-875 trigge

SUBMITTER: Goldspink DA 

PROVIDER: S-EPMC5784317 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature