Purification and characterization of fibrinolytic enzyme from a bacterium isolated from soil.

ABSTRACT: A novel extracellular enzyme with strong fibrinolytic activity, produced by Bacillus tequilensis, which was isolated from the soil of Zhuhai City (China) was purified and characterized. The enzyme was secreted by cultured B. tequilensis in solid state and purified at a high efficiency using the combination of salting out, ion exchange chromatography, and size exclusion chromatography. The enzyme was estimated to have a molecular weight of approximately 27 kDa, pI of 8.9 ± 0.1, to stable at pH 5.0-12.0 and up to 50 °C; the optimum pH and temperature are 10.5 and 45 °C (2373.59 ± 54.81 U/mg), respectively. The fibrinolytic activity was enhanced by K⁺, Na⁺, Mg²⁺, Mn²⁺, Ca²⁺, and Ba²⁺ and inhibited by Cu²⁺

SUBMITTER: Xin X 

PROVIDER: S-EPMC5796941 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature