Large-scale comparative epigenomics reveals hierarchical regulation of non-CG methylation in Arabidopsis.

ABSTRACT: Genome-wide characterization by next-generation sequencing has greatly improved our understanding of the landscape of epigenetic modifications. Since 2008, whole-genome bisulfite sequencing (WGBS) has become the gold standard for DNA methylation analysis, and a tremendous amount of WGBS data has been generated by the research community. However, the systematic comparison of DNA methylation profiles to identify regulatory mechanisms has yet to be fully explored. Here we reprocessed the raw data of over 500 publicly available Arabidopsis WGBS libraries from various mutant backgrounds, tissue types, and stress treatments and also filtered them based on sequencing depth and efficiency of bisulfite conversion. This enabled us to identify high-confidence differentially methylated regions

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5798360 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature