Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING).

ABSTRACT: Imaging and chromatin capture techniques have provided important insights into our understanding of nuclear organization. A limitation of these techniques is the inability to resolve allele-specific spatiotemporal properties of genomic loci in living cells. Here, we describe an allele-specific CRISPR live-cell DNA imaging technique (SNP-CLING) to provide the first comprehensive insights into allelic positioning across space and time in mouse embryonic stem cells and fibroblasts. With 3D imaging, we studied alleles on different chromosomes in relation to one another and relative to nuclear substructures such as the nucleolus. We find that alleles maintain similar positions relative to each other and the nucleolus; however, loci occupy unique positions. To monitor spatiotemporal dynamics by

SUBMITTER: Maass PG 

PROVIDER: S-EPMC5805655 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature