Library Design-Facilitated High-Throughput Sequencing of Synthetic Peptide Libraries.

ABSTRACT: A methodology to achieve high-throughput de novo sequencing of synthetic peptide mixtures is reported. The approach leverages shotgun nanoliquid chromatography coupled with tandem mass spectrometry-based de novo sequencing of library mixtures (up to 2000 peptides) as well as automated data analysis protocols to filter away incorrect assignments, noise, and synthetic side-products. For increasing the confidence in the sequencing results, mass spectrometry-friendly library designs were developed that enabled unambiguous decoding of up to 600 peptide sequences per hour while maintaining greater than 85% sequence identification rates in most cases. The reliability of the reported decoding strategy was additionally confirmed by matching fragmentation spectra for select authentic peptides identi

SUBMITTER: Vinogradov AA 

PROVIDER: S-EPMC5818986 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature