Pervasive, Coordinated Protein-Level Changes Driven by Transcript Isoform Switching during Meiosis.

ABSTRACT: To better understand the gene regulatory mechanisms that program developmental processes, we carried out simultaneous genome-wide measurements of mRNA, translation, and protein through meiotic differentiation in budding yeast. Surprisingly, we observed that the levels of several hundred mRNAs are anti-correlated with their corresponding protein products. We show that rather than arising from canonical forms of gene regulatory control, the regulation of at least 380 such cases, or over 8% of all measured genes, involves temporally regulated switching between production of a canonical, translatable transcript and a 5' extended isoform that is not efficiently translated into protein. By this pervasive mechanism for the modulation of protein levels through a natural developmental program, a si

SUBMITTER: Cheng Z 

PROVIDER: S-EPMC5826577 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature