Dataset Information

Characterization and transferability of microsatellites for Gentiana lawrencei var. farreri (Gentianaceae).

ABSTRACT:

Premise of the study

Microsatellite markers were developed for a medicinal herb, Gentiana lawrencei var. farreri (Gentianaceae), for the future assessment of population genetic structure and potential hybridization events with related taxa.

Methods and results

Using the 454 FLX+ sequencing platform, we obtained 81,717 clean reads with an average length of 291 bp. A total of 3031 primer pairs were designed, and 96 were selected for validation. A set of 20 fluorescently labeled primer pairs was further selected and screened for polymorphisms in three G. lawrencei var. farreri populations and one G. veitchiorum population. Among the four populations, the average number of alleles per locus was 15.2. Finally, a set of 17 unlinked loci were determined to be in Hardy-Weinberg equilibrium after two linked loci were removed.

Conclusions

The identified simple sequence repeat markers will be useful for genetic diversity and evolution studies in G. lawrencei var. farreri and related taxa.

SUBMITTER: Sun SS

PROVIDER: S-EPMC5828123 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Publications

Characterization and transferability of microsatellites for <i>Gentiana lawrencei</i> var. <i>farreri</i> (Gentianaceae).

Sun Shan-Shan SS Fu Peng-Cheng PC Cheng Yan-Wei YW Zhou Xiao-Jun XJ Han Jian-Min JM

Applications in plant sciences 20180101 1

<h4>Premise of the study</h4>Microsatellite markers were developed for a medicinal herb, <i>Gentiana lawrencei</i> var. <i>farreri</i> (Gentianaceae), for the future assessment of population genetic structure and potential hybridization events with related taxa.<h4>Methods and results</h4>Using the 454 FLX+ sequencing platform, we obtained 81,717 clean reads with an average length of 291 bp. A total of 3031 primer pairs were designed, and 96 were selected for validation. A set of 20 fluorescentl ...[more]

PMID: 29732246

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