Characterization and transferability of microsatellites for Gentiana lawrencei var. farreri (Gentianaceae).

ABSTRACT:

Premise of the study

Microsatellite markers were developed for a medicinal herb, Gentiana lawrencei var. farreri (Gentianaceae), for the future assessment of population genetic structure and potential hybridization events with related taxa.

Methods and results

Using the 454 FLX+ sequencing platform, we obtained 81,717 clean reads with an average length of 291 bp. A total of 3031 primer pairs were designed, and 96 were selected for validation. A set of 20 fluorescently labeled primer pairs was further selected and screened for polymorphisms in three G. lawrencei var. farreri populations and one G. veitchiorum population. Among the four populations, the average number of alleles per locus was 15.2. Finally, a set of 17 unlinked loci were dete

SUBMITTER: Sun SS 

PROVIDER: S-EPMC5828123 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature