Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain.

ABSTRACT: DNA methylation is an important epigenetic modification involved in gene regulation and transposable element silencing. Changes in DNA methylation can be heritable and, thus, can lead to the formation of stable epialleles. A well-characterized example of a stable epiallele in plants is fwa, which consists of the loss of DNA cytosine methylation (5mC) in the promoter of the FLOWERING WAGENINGEN (FWA) gene, causing up-regulation of FWA and a heritable late-flowering phenotype. Here we demonstrate that a fusion between the catalytic domain of the human demethylase TEN-ELEVEN TRANSLOCATION1 (TET1cd) and an artificial zinc finger (ZF) designed to target the FWA promoter can cause highly efficient targeted demethylation, FWA up-regulation, and a heritabl

SUBMITTER: Gallego-Bartolome J 

PROVIDER: S-EPMC5834696 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature