Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

ABSTRACT:

Background

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are providing new possibilities for the biological study, cell therapies, and drug discovery. However, the ion channel expression and functions as well as regulations in hiPSC-CMs still need to be fully characterized.

Methods

Cardiomyocytes were derived from hiPS cells that were generated from two healthy donors. qPCR and patch clamp techniques were used for the study.

Results

In addition to the reported ion channels, I_Na, I_Ca-L, I_Ca-T, I_f, I_NCX, I_K1, I_to, I_Kr, I_Ks I_KATP, I_K-pH, I_SK1-3, and I_SK4, we detected both the expression and currents of ACh

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC5835237 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature