A novel model to characterize structure and function of BRCA1.

ABSTRACT: BRCA1 plays a central role in DNA repair. Although N-terminal RING and C-terminal BRCT domains are studied well, the functions of the central region of BRCA1 are poorly characterized. Here, we report a structural and functional analysis of BRCA1 alleles and functional human BRCA1 in chicken B-lymphocyte cell line DT40. The combination of "homologous recombineering" and "RT-cassette" enables modifications of chicken BRCA1 gene in Escherichia coli. Mutant BRCA1 knock-in DT40 cell lines were generated using BRCA1 mutation constructs by homologous recombination with a targeting efficiency of up to 100%. Our study demonstrated that deletion of motifs 2-9 BRCA1^{Δ/Δ181-1415} (Caenorhabditis elegans BRCA1 mimic) or deletion of motif 1 BRCA1^Δ/Δ126-136 decreased cell viability fo

SUBMITTER: Lin D 

PROVIDER: S-EPMC5839108 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature