Stem cell properties of human clonal salivary gland stem cells are enhanced by three-dimensional priming culture in nanofibrous microwells.

ABSTRACT:

Background

Three-dimensional (3D) cultures recapitulate the microenvironment of tissue-resident stem cells and enable them to modulate their properties. We determined whether salivary gland-resident stem cells (SGSCs) are primed by a 3D spheroid culture prior to treating irradiation-induced salivary hypofunction using in-vitro coculture and in-vivo transplant models.

Methods

3D spheroid-derived SGSCs (SGSCs^3D) were obtained from 3D culture in microwells consisting of a nanofiber bottom and cell-repellent hydrogel walls, and were examined for salivary stem or epithelial gene/protein expression, differentiation potential, and paracrine secretory function compared with monolayer-cultured SGSCs (SGSCs^2D) in vitro and in vivo.

Results

SGSCs^3D

SUBMITTER: Shin HS 

PROVIDER: S-EPMC5863805 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature