Metabolomic approach for a rapid identification of natural products with cytotoxic activity against human colorectal cancer cells.

ABSTRACT: The discovery of bioactive compounds from natural sources entails an extremely lengthy process due to the timescale and complexity of traditional methodologies. In our study, we used a rapid NMR based metabolomic approach as tool to identify secondary metabolites with anti-proliferative activity against a panel of human colorectal cancer cell lines with different mutation profiles. For this purpose, fourteen Fabaceae species of Mediterranean vegetation were investigated using a double screening method: ¹H NMR profiling enabled the identification of the main compounds present in the mixtures, whilst parallel biological assays allowed the selection of two plant extracts based on their strong anti-proliferative properties. Using high-resolution 2D NMR spectroscopy, putative active

SUBMITTER: Graziani V 

PROVIDER: S-EPMC5871890 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature