Transcriptome and secretome analysis of Aspergillus fumigatus in the presence of sugarcane bagasse.

ABSTRACT:

Background

Sugarcane bagasse has been proposed as a lignocellulosic residue for second-generation ethanol (2G) produced by breaking down biomass into fermentable sugars. The enzymatic cocktails for biomass degradation are mostly produced by fungi, but low cost and high efficiency can consolidate 2G technologies. A. fumigatus plays an important role in plant biomass degradation capabilities and recycling. To gain more insight into the divergence in gene expression during steam-exploded bagasse (SEB) breakdown, this study profiled the transcriptome of A. fumigatus by RNA sequencing to compare transcriptional profiles of A. fumigatus grown on media containing SEB or fructose as the sole carbon source. Secretome analysis was also performed using SDS-PAGE and LC-MS/MS.

Results

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SUBMITTER: de Gouvea PF 

PROVIDER: S-EPMC5883313 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature