Transgenic Xenopus laevis Line for In Vivo Labeling of Nephrons within the Kidney.

ABSTRACT: Xenopus laevis embryos are an established model for studying kidney development. The nephron structure and genetic pathways that regulate nephrogenesis are conserved between Xenopus and humans, allowing for the study of human disease-causing genes. Xenopus embryos are also amenable to large-scale screening, but studies of kidney disease-related genes have been impeded because assessment of kidney development has largely been limited to examining fixed embryos. To overcome this problem, we have generated a transgenic line that labels the kidney. We characterize this cdh17:eGFP line, showing green fluorescent protein (GFP) expression in the pronephric and mesonephric kidneys and colocalization with known kidney markers. We also demonstrate the feasibility of live

SUBMITTER: Corkins ME 

PROVIDER: S-EPMC5924539 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature