Energetics underlying hemin extraction from human hemoglobin by Staphylococcus aureus.

ABSTRACT: Staphylococcus aureus is a leading cause of life-threatening infections in the United States. It actively acquires the essential nutrient iron from human hemoglobin (Hb) using the iron-regulated surface-determinant (Isd) system. This process is initiated when the closely related bacterial IsdB and IsdH receptors bind to Hb and extract its hemin through a conserved tri-domain unit that contains two NEAr iron Transporter (NEAT) domains that are connected by a helical linker domain. Previously, we demonstrated that the tri-domain unit within IsdH (IsdH^N2N3) triggers hemin release by distorting Hb's F-helix. Here, we report that IsdH^N2N3 promotes hemin release from both the α- and β-subunits. Using a receptor mutant that only binds to the α-subunit of Hb and a stop

SUBMITTER: Sjodt M 

PROVIDER: S-EPMC5936817 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature