Quantitative proteomic profiling of primary cancer-associated fibroblasts in oesophageal adenocarcinoma.

ABSTRACT:

Background

Cancer-associated fibroblasts (CAFs) form the major stromal component of the tumour microenvironment (TME). The present study aimed to examine the proteomic profiles of CAFs vs. normal fibroblasts (NOFs) from patients with oesophageal adenocarcinoma to gain insight into their pro-oncogenic phenotype.

Methods

CAFs/NOFs from four patients were sub-cultured and analysed using quantitative proteomics. Differentially expressed proteins (DEPs) were subjected to bioinformatics and compared with published proteomics and transcriptomics  datasets.

Results

Principal component analysis of all profiled proteins showed that CAFs had high heterogeneity and clustered separately from NOFs. Bioinformatics interrogation of the DEPs demonstrated inhibition of adhesion of epit

SUBMITTER: Manousopoulou A 

PROVIDER: S-EPMC5943522 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature