Mango: A General Tool for Collision Induced Dissociation-Cleavable Cross-Linked Peptide Identification.

ABSTRACT: Chemical cross-linking combined with mass spectrometry provides a method to study protein structures and interactions. The introduction of cleavable bonds in a cross-linker provides an avenue to decouple released peptide masses from their precursor species, greatly simplifying the downstream search, allowing for whole proteome investigations to be performed. Typically, these experiments have been challenging to carry out, often utilizing nonstandard methods to fully identify cross-linked peptides. Mango is an open source software tool that extracts precursor masses from chimeric spectra generated using cleavable cross-linkers, greatly simplifying the downstream search. As it is designed to work with chimeric spectra, Mango can be used on traditional high-resolution tandem mass spectrometry

SUBMITTER: Mohr JP 

PROVIDER: S-EPMC5959040 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature