Defining the Transcriptional Targets of Leptin Reveals a Role for Atf3 in Leptin Action.

ABSTRACT: Leptin acts via its receptor (LepRb) to modulate gene expression in hypothalamic LepRb-expressing neurons, thereby controlling energy balance and glucose homeostasis. Despite the importance of the control of gene expression in hypothalamic LepRb neurons for leptin action, the transcriptional targets of LepRb signaling have remained undefined because LepRb cells contribute a small fraction to the aggregate transcriptome of the brain regions in which they reside. We thus employed translating ribosome affinity purification followed by RNA sequencing to isolate and analyze mRNA from the hypothalamic LepRb neurons of wild-type or leptin-deficient (Lep^ob/ob) mice treated with vehicle or exogenous leptin. Although the expression of most of the genes encoding the neuropeptides co

SUBMITTER: Allison MB 

PROVIDER: S-EPMC5961413 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature