Cardiac Kir2.1 and Na_V1.5 Channels Traffic Together to the Sarcolemma to Control Excitability.

ABSTRACT:

Rationale

In cardiomyocytes, Na_V1.5 and Kir2.1 channels interact dynamically as part of membrane bound macromolecular complexes.

Objective

The objective of this study was to test whether Na_V1.5 and Kir2.1 preassemble during early forward trafficking and travel together to common membrane microdomains.

Methods and results

In patch-clamp experiments, coexpression of trafficking-deficient mutants Kir2.1^Δ314-315 or Kir2.1^R44A/R46A with wild-type (WT) Na_V1.5^WT in heterologous cells reduced inward sodium current compared with Na_V1.5^WT alone or coexpressed with Kir2.1^WT. In cell surface biotinylation experiments, expression of Kir2.1^Δ314-315 reduced Na_V1.5

SUBMITTER: Ponce-Balbuena D 

PROVIDER: S-EPMC5970051 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature