Enhanced ZnR/GPR39 Activity in Breast Cancer, an Alternative Trigger of Signaling Leading to Cell Growth.

ABSTRACT: Acquired resistance to the estrogen receptor (ER) antagonist tamoxifen, is a major obstacle in treatment of breast cancer. Changes in Zn²⁺ accumulation and distribution are associated with tamoxifen-resistance and breast cancer progression. The Zn²⁺-sensing G-protein coupled receptor, ZnR/GPR39, triggers signaling leading to cell growth, but a role for this receptor in breast cancer in unknown. Using fluorescence imaging, we found Zn²⁺-dependent Ca²⁺ release, mediated by ZnR/GPR39 activity, in TAMR tamoxifen-resistant cells derived from MCF-7 cells, but not in ER-expressing MCF-7 or T47D cells. Furthermore, ZnR/GPR39 signaling was monitored in ER negative BT20, MDA-MB-453 and JIMT-1 cells. Expression of ZnR/GPR39 was increased in grade 3 human br

SUBMITTER: Ventura-Bixenshpaner H 

PROVIDER: S-EPMC5970167 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature