Unknown

Dataset Information

0

Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections.


ABSTRACT: Blackcurrant leaf chlorosis associated virus (BCLCaV) was detected recently by next-generation sequencing (NGS) and a new and distinct species in the genus Idaeovirus was proposed. Analysis of NGS-derived paired-end reads revealed the existence of bridge reads encompassing the 3'-terminus and 5'-terminus of RNA-2 or RNA-3 of BCLCaV. The full RNA-2 or RNA-3 could be amplified using outward facing or abutting primers; also, RNA-2/RNA-3 could be detected even after three consecutive RNase R enzyme treatments, with denaturation at 95 °C preceding each digestion. Evidence was obtained indicating that there are circular forms of BCLCaV RNA-2 and RNA-3.

SUBMITTER: James D 

PROVIDER: S-EPMC5977253 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections.

James Delano D   Phelan James J   Sanderson Daniel D  

Viruses 20180515 5


Blackcurrant leaf chlorosis associated virus (BCLCaV) was detected recently by next-generation sequencing (NGS) and a new and distinct species in the genus <i>Idaeovirus</i> was proposed. Analysis of NGS-derived paired-end reads revealed the existence of bridge reads encompassing the 3'-terminus and 5'-terminus of RNA-2 or RNA-3 of BCLCaV. The full RNA-2 or RNA-3 could be amplified using outward facing or abutting primers; also, RNA-2/RNA-3 could be detected even after three consecutive RNase R  ...[more]

Similar Datasets

| S-EPMC10589165 | biostudies-literature
| S-EPMC6834158 | biostudies-literature
| S-EPMC9194764 | biostudies-literature
| S-EPMC6171863 | biostudies-literature
| S-EPMC7238886 | biostudies-literature
| S-EPMC1553207 | biostudies-literature
| S-EPMC10086252 | biostudies-literature
| S-EPMC5985646 | biostudies-literature
| S-EPMC8201402 | biostudies-literature
| S-EPMC7083127 | biostudies-literature