Ontology highlight
ABSTRACT:
SUBMITTER: James D
PROVIDER: S-EPMC5977253 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
James Delano D Phelan James J Sanderson Daniel D
Viruses 20180515 5
Blackcurrant leaf chlorosis associated virus (BCLCaV) was detected recently by next-generation sequencing (NGS) and a new and distinct species in the genus <i>Idaeovirus</i> was proposed. Analysis of NGS-derived paired-end reads revealed the existence of bridge reads encompassing the 3'-terminus and 5'-terminus of RNA-2 or RNA-3 of BCLCaV. The full RNA-2 or RNA-3 could be amplified using outward facing or abutting primers; also, RNA-2/RNA-3 could be detected even after three consecutive RNase R ...[more]