Nasal DNA methylation is associated with childhood asthma.

ABSTRACT:

Aim

We aim to study DNA methylation (DNAm) variations associated with childhood asthma.

Methods

Nasal DNAm was compared between sibling pairs discordant for asthma, 29 sib pairs for genome-wide association studies and 54 sib pairs for verification by pyrosequencing. Associations of methylation with asthma symptoms, allergy and environmental exposures were evaluated. In vitro experiments and functional genomic analyses were performed to explore biologic relevance.

Results

Three CpGs were associated with asthma. cg14830002 was associated with allergies in nonasthmatics. cg23602092 was associated with asthma symptoms. cg14830002 and cg23602092 were associated with traffic-related air pollution exposure. Nearby genes were transcriptionally regulated by diesel exhaust, hou

SUBMITTER: Zhang X 

PROVIDER: S-EPMC5992571 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature