Use of a leukocyte-targeted peptide probe as a potential tracer for imaging the tuberculosis granuloma.

ABSTRACT: Granulomas are the histopathologic hallmark of tuberculosis (TB), both in latency and active disease. Diagnostic and therapeutic strategies that specifically target granulomas have not been developed. Our objective is to develop a probe for imaging relevant immune cell populations infiltrating the granuloma. We report the binding specificity of Cyanine 3 (Cy3)-labeled cFLFLFK-PEG₁₂ to human leukocytes and cellular constituents within a human in vitro granuloma model. We also report use of the probe in in vivo studies using a mouse model of lung granulomatous inflammation. We found that the probe preferentially binds human neutrophils and macrophages in human granuloma structures. Inhibition studies showed that peptide binding to human neutrophils is mediated by the receptor form

SUBMITTER: Locke LW 

PROVIDER: S-EPMC6014630 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature