Modulating cell state to enhance suspension expansion of human pluripotent stem cells.

ABSTRACT: The development of cell-based therapies to replace missing or damaged tissues within the body or generate cells with a unique biological activity requires a reliable and accessible source of cells. Human pluripotent stem cells (hPSC) have emerged as a strong candidate cell source capable of extended propagation in vitro and differentiation to clinically relevant cell types. However, the application of hPSC in cell-based therapies requires overcoming yield limitations in large-scale hPSC manufacturing. We explored methods to convert hPSC to alternative states of pluripotency with advantageous bioprocessing properties, identifying a suspension-based small-molecule and cytokine combination that supports increased single-cell survival efficiency, faster growth rates, higher densities, and grea

SUBMITTER: Lipsitz YY 

PROVIDER: S-EPMC6016797 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature