In Vitro Studies of Lipopolysaccharide-Mediated DNA Release of Podovirus HK620.

ABSTRACT: Gram-negative bacteria protect themselves with an outermost layer containing lipopolysaccharide (LPS). O-antigen-specific bacteriophages use tailspike proteins (TSP) to recognize and cleave the O-polysaccharide part of LPS. However, O-antigen composition and structure can be highly variable depending on the environmental conditions. It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance. In this work, we have analyzed how LPS preparations in vitro trigger particle opening and DNA ejection from the E. coli podovirus HK620. Fluorescence-based monitoring of DNA release showed that HK620 phage particles in vitro ejected their genome at velocities

SUBMITTER: Broeker NK 

PROVIDER: S-EPMC6024685 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature