Isolation and characterization of microsatellite loci for Prunus mongolica (Rosaceae).

ABSTRACT:

Premise of the study

Microsatellite primers were developed in Prunus mongolica (Rosaceae), a relict flora endemic in arid areas of the Asian interior, to investigate the genetic diversity, phylogeography, population structure, and history of the species.

Methods and results

Fifty-one microsatellite loci, including di-, tri-, and tetranucelotide repeats, were identified using transcriptome sequencing and bioinformatic screening. The number of alleles ranged from seven to 11 and the levels of observed and expected heterozygosity ranged from 0.545 to 1.000 and 0.600 to 0.989, respectively. Most of the primers also amplified in a group of congeneric species (P. triloba, P. davidiana, P. persica, P. cerasifera, and P. serrulata).

Concl

SUBMITTER: Cheng YC 

PROVIDER: S-EPMC6025809 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature